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The tube was capped and shaken vertically for 5 min at 1500 strokes/min in an automatic pulverizer (Automill, Tokken). After brief centrifugation, the bullet was removed with tweezers and the sorbent of Oasis MAX (30 μm in diameter, Waters, Milford, MA, USA; 3 mg/mg hair) added to adsorb anionic interferences.

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Seed samples were ground for 1 min at 1500 strokes per min with an automatic pulverizer (Automill TK-AM5, Tokken Inc., Chiba, Japan).

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The tube was capped and shaken vertically for 5 min at 1500 strokes/min with an automatic pulverizer (Automill, Tokken). A total of 12 tubes could be processed in parallel. After a brief centrifugation, the bullet was removed with tweezers and the tube centrifuged again for 2 min. The supernatant was filtered using a centrifugal filter unit ...

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The tube was capped and shaken vertically for 5 min at 1500 strokes/min with an automatic pulverizer (Automill, Tokken). A total of 12 tubes could be processed in parallel. After a brief centrifugation, the bullet was removed with tweezers and the tube centrifuged again for 2 min.

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2 Bio-protocol 10(09): e3613. DOI:10.21769/BioProtoc.3613 Copyright Hamano et al . This article is distributed under the terms of the ...

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